A High-Throughput Arabidopsis Reverse Genetics System
نویسندگان
چکیده
منابع مشابه
A high-throughput Arabidopsis reverse genetics system.
A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymmetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from approximately 100000 transformed lines. A total of 85108 TAIL-PCR products from 52964 T-DNA lines w...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 2002
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.004630